POLYPHENOL EFFECT TO INSULIN RECEPTOR-β EXPRESSION ON THE SCELETAL MUSCLE OF DIABETIC-INDUCED MICE

POLYPHENOL EFFECT TO INSULIN RECEPTOR-β EXPRESSION ON THE SCELETAL MUSCLE OF DIABETIC-INDUCED MICE

Hesti Riany1, Ahmad Ridwan2, Anggraini Barlian2

1hesti.riany@unja.ac.id

School of Life Science and Technology—Institut Teknologi Bandung

Abstract

In diabetes, hyperglycemia can increase ROS production and accelerate diabetes complication by some defects in cell signaling include Insulin receptor-β (IRβ). Polyphenol from green tea has effect to decrease ROS level. In this study, mice (ddY) were induced become diabetic by intra venous injection of Alloxan doses 70 mg/kg body weight. Diabetic mice with blood glucose ≥ 200 mg/dl were used. Mice were divided into three groups, there were normal group, diabetic mice were given control solution know as (P0) and diabetic mice were given 100 mg/kg body weight of polyphenol (P100). Each animal were given control solution and polyphenol daily 22 days. IRβ protein were isolated from muscle tissue and then the expression were observed by using Western Blotting then were examined by using enhanced chemiluminescene. This study shown that IRβ expression from all group based on condition (fasting, 1 hour PP and 2 hour PP) have shown two expressions. First, in 1 hour PP indicated a tendency that P0 and P100 IRβ expressions were lower than normal mice and P100 was the lowest. Second, in the fasting and 2 hour PP indicated a tendency that P0 and P100 IRβ expressions were higher than normal mice and P100 was the highest.

Keywords: Polyphenol, IRβ, antioxidant, ROS, Alloxan