Daya Hambat Detergen Cair terhadap Pertumbuhan Bakteri pada Pakaian Bekas dari Arizona Kota Jambi
Liquid Detergent Inhibition to growth of Bacteria of secondhand clothes from Arizona, Jambi City
Hesti Riany1), Hasnaul Maritsa2)
1)Fakultas Sains dan Teknologi, Universitas Jambi
Email: hestiriany@unja.ac.id
2)Fakultas Sains dan Teknologi, Universitas Jambi
Email: hasnaul.maritsa123@gmail.com
Abstrak
Peminatan pakaian bekas, terutama pakaian bekas impor di kota Jambi cukup tinggi. Padahal penggunaan pakaian bekas impor telah dilarang oleh Kementerian Perdagangan karena ditemukan ribuan koloni mikroba dan ribuan koloni jamur. Selain itu impor pakaian bekas merugikan negara karena masuk ke Indonesia secara ilegal., Tujuan penelitian ini adalah untuk mengetahui daya hambat detergen cair terhadap bakteri pada pakaian bekas yang dijual di kawasan Arizona kota Jambi. Bakteri yang digunakan diisolasi dari empat sampel baju bekas impor dari empat lokasi dan 1 sampel baju bekas harian sebagai pembanding. Bakteri yang berbeda pada setiap sampel diisolasi, dan didapatkan 11 isolat. Kemudian Isolat tersebut dikarakterisasi dan digunakan untuk uji daya hambat mengunakan detergen cair dengan konsentrasi 0%, 1% dan 10%. Data yang diperoleh berupa diameter zona hambat yang selanjutnya dianalisis dengan ANNOVA. Hasil penelitian menunjukkan semua bakteri berbentuk kokus. Uji gram menunjukkan 9 isolat merupakan bakteri gram negatif dan 2 isolat bakteri gram positif. Pemberian detergen 10 % pada setiap isolat memberikan pengaruh berbeda nyata terhadap pertumbuhan bakteri sedangkan pemberian detergen 1 % tidak memberikan pengaruh nyata terhadap pertumbuhan bakteri.Abstrak
Kata Kunci: detergen cair, pakaian bekas, bakteri, Jambi
Abstract
Interesting in secondhand clothes, especially imported secondhand clothes in Jambi city is quite high. Whereas the using of imported secondhand clothes had been banned by the Ministry of Trade because there were thousands of microbial colonies and thousands of fungal colonies have been discovered on its. Besides, the secondhand clothes caused unprofitable to country that entered Indonesia illegally. The aim of this study was to determine the liquid detergent inhibition to growth of bacteria of secondhand clothes that sold in the Arizona region, Jambi city. The bacteria were isolated from four samples of imported secondhand clothes from four locations and one sample daily used clothes for comparison. Different bacteria in each sample were isolated, and obtained 11 isolates. Then Isolates were characterized and used to test the liquid detergent inhibition ability that used 0%, 1% and 10% in concentrations. Inhibition zone data that obtained as diameters were analyzed by ANOVA. The results showed all bacteria were cocci shaped. Gram staining showed that 9 isolates were gram-negative bacteria and 2 isolates were gram-positive bacteria. Treatment using 10 % detergent showed significantly effect on bacterial growth in each isolat, while 1% detergent did not give a significant effect on the bacteria growth.
Keywords: liquid detergent, secondhand clothes, bacteria, Jambi
